Lactophenol, staining solutions, by loba chemie, india. Identification of the substancemixture and of the companyundertaking 1. Safety data sheet lactophenol cotton blue according to regulation ec no 19072006, annex ii, as amended by regulation eu no 4532010 section 1. P ehrlich was among the first to study systematically synthetic dyes and to introduce scientific methods into the field of. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. Jorvet j326b lactophenol cotton blue stain for animal use. Bbl lactophenol cotton blue stain droppers are used in wet amounts for examination of fungi 50sp, 500ca.
The cotton blue is a histological stain which turns the fungal cell walls blue. Aug 17, 2012 short video of using a lactophenol cotton blue stain for identifying fungal elements on a glass slide. New staining technique for fungalinfected plant tissues. Product identifier product name lactophenol cotton blue product number pl. Page 17 safety data sheet according to whs regulations printing date 29. Cotton blue is an acid dye that stains the chitin present. Lactophenol cotton blue lpcb for the staining and microscopic identification of fungi. Feb 03, 2015 staining of volutincontaining organisms well developed granules of volutin polyphosphate may be seen in unstained wet preparation as round refractile bodies within the bacterial cytoplasm. This item is sold and shipped to schools and businesses only. Lactophenol anilin blue solution, lactophenol cotton blue solution.
Acid fast staining technique and its details in english. Staining of fungal cells by using lactophenol cotton blue. Methyl blue is a chemical compound with the molecular formula c 37 h 27 n 3 na 2 o 9 s 3. Lactophenol blue solution for microscopy, for staining molds. Lactophenol cotton blue stain fungal element stain for mycology. License creative commons attribution license reuse. This product is intended to be used primarily with pure cultures, although certain specimens. The lactophenol cotton blue lpcb wet mount preparation is the most widely used method of staining and observing fungi and is simple to prepare. Aniline blue fluorescence has been widely used in botanical histochemistry, particularly to stain callose plugs in phloem 14. Lpcb100, lpcb500, lpcba100, lpcba500 recommended use of the chemical and restriction on use. It is used as a stain in histology, and stains collagen blue in tissue sections. Add one or two drops of lactophenol cotton blue stain before alcohol gets off. Blue g250based protein stain, gelcode blue stain reagent, which uses the colloidal properties of coomassie g250 for protein staining in polyacrylamide gels. Sigmaaldrich 3050 spruce street saint louis mo 63103 usa telephone.
Staining of clinical specimens nonkeratinized procedure lcb staining 1. Lactophenol cotton blue microbiology differentiation aids. Lactophenol cotton blue stain medical laboratories. Preparation of lactophenol cotton blue slide mounts. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. What safe alternatives are there to lactophenol cotton blue.
For example, only the protein is stained, not the gel. The slides were analysed, and the images were captured with a leica dc 300f digital camera attached to a leica dmld microscope. An aniline blue staining procedure for confocal microscopy and 3d imaging of normal and perturbed cellular phenotypes in mature arabidopsis embryos sue bougourd1, jo marrison1 and jim haseloff2 1department of biology, university of york, po box 373, york yo10 5yw, uk, and. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. This exclusive pierce formulation yields staining properties unique to a coomassie brilliant blue gbased protein stain. Lactic acid acts as a preservative and it preserves the structure of fungus. The material is stained in one step with lactophenol blue solution. Clin microbiology stains, media and identification questions. Wet mount preparation is the most widely used method of. Short video of using a lactophenol cotton blue stain for identifying fungal elements on a glass slide.
Slide culture technique and lactophenol cotton blue staining. Add one or two drops of l actophenol cotton blue stain before alcohol gets off. To perform tests, squeeze the dropper to crush the ampule. Start studying clin microbiology stains, media and identification. Lactophenol cotton blue stain fungal element stain for. Our lactophenol blue stain is used as a mounting medium for studying fungi. Lactophenol cotton blue stain other means of identification. Lactophenol cotton blue stain of penicillium species.
Staining techniques in microbiology linkedin slideshare. Lactophenol along with cotton blue reagent is used for staining as well as for wet mounting of fungi1. This readytouse solution is especially for airdried, heatfixed smears of bacteriological material, e. Lactophenol cotton blue used for wet mounts in the examination of yeasts and molds, serving as mounting fluid and stain. With basic dyes they tend to stain more strongly than the rest of the bacterium, and with toluidine blue or methylene blue they stain. I am not very sure what it does in lactophenol cotten blue, but can speculate that it helps in retaining dye cotton blue in the fungal cell wall. Lactophenol cotton blue stain is recommended for mounting and staining yeast and molds. Staining of fungus by using lactophenol cotton blue. Page 47 safety data sheet according to whs regulations printing date 29. Invert the dropper for convenient dropbydrop dispensing. Intended use hardy diagnostics bluemount is recommended for the permanent mounting and staining of yeasts and molds.
Jan 20, 2016 jan 20, 2016 lactophenol cotton blue stain of penicillium species. Place a drop of seventy percent alcohol on a clean microscope slide. Iodineglycerol as an alternative to lactophenol cotton blue for identification of fungal elements in clinical laboratory. Acid fuchsin and cotton blue can also be used to stain fungi in roots, but destain rapidly and produce lowcontrast images brundrett et al. License creative commons attribution license reuse allowed. Although the common name of this stain varies with the author or source i. An aniline blue staining procedure for confocal microscopy. Lpcb is a stain used for making semipermanent microscopic preparation of fungi. Learn vocabulary, terms, and more with flashcards, games, and other study tools. This is a compound stain formed by interaction of methylene blue and eosin. Innumerable stains have been tried for differentiating between parasite and host, and undoubtedly a combination of lactophenol and cotton blue is one of the best stains for general work. It is a wet mount preparation fungal samples are simply transferred onto a slide and viewed wet. Comparison of microscopic morphology of fungi using. Summary hardy diagnostics bluemount is a formulation of lactophenol cotton blue with polyvinyl alcohol and is recommended for preparing permanent, longlasting mounts of fungal wet preparations or slide cultures.
The modified method ie maygrunwald giemsa mgg technique is. This gives excellent results with hand sections and bulk. Preparation of lactophenol cotton blue lpcb slide mounts. Preparation of lactophenol cotton blue slide mounts ncbi. Bluemount mycology stain and permanent slide mounting. Lactophenol blue stain is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. P ehrlich was among the first to study systematically synthetic dyes and to introduce scientific methods into the field of histological dye staining ehrlich p, 1877, 1878. Organisms suspended in the stain are killed due to the presence of phenol. Lactophenol cotton blue stain definition of lactophenol. Specimen mounts for microscopic examination of moulds includes teased, mashed, and slide culture preparations. Wet mount staining do not allow observation of early stage differentiation of the fungus. On exposure to acids, alkali and ultraviolet light, a number of oxidation products methylene azures are formed from methylene blue which give contrast staining. For the staining and microscopic identification of fungi.
It can be used in some differential staining techniques such as mallorys connective tissue stain and gomori trichrome stain, and can be used to mediate electron transfer in microbial fuel cells. Immediately flush eyes with gentle but large stream of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Learn about jorvet j326b lactophenol cotton blue stain for animal usage including. Eng scientific lactophenol cotton blue stain, certified. Staining of volutincontaining organisms well developed granules of volutin polyphosphate may be seen in unstained wet preparation as round refractile bodies within the bacterial cytoplasm. Wet mount can not be stored over longer period of time. Here in this mounting method we use lactophenol cotton blue stain where lactophenol acts as a cleaning agent as phenol is fungicidal in nature so it kills the organism. Aug 09, 2017 staining of fungal cells by using lactophenol cotton blue microbiology with sumi. Jan 20, 2016 lactophenol cotton blue stain of penicillium species. Jul 20, 2014 2 r vignesh, cr swathirajan, s solomon, em shankar, kg murugavel, i paul, et al. Staining of fungal cells by using lactophenol cotton blue microbiology with sumi. The modified method ie maygrunwald giemsa mgg technique is commonly used.
Use of safe work practices are recommended to avoid eye or skin contact and inhalation of vapours. Stain fungal elements with thermo scientific remel lactophenol aniline blue. Dropper contains oneday supply of reagent or stain, hermetically sealed inside an ampule. Can anyone identify these fungal structures stained with. The lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi.
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